Spinal Cord Transection In <em>Xenopus laevis</em> Tadpoles

Spinal cord injury (SCI) is a permanent affliction, which affects the central nervous system (CNS) motor and sensory nerves, resulting in paralysis beneath site. To date, there no functional recovery therapy for SCI, lack of clarity regarding many complexes dynamic events occurring after SCI. Many non-mammalian organisms can regenerate severe such as teleost fishes, urodele amphibians, larval stages anuran including Xenopus laevis tadpoles. These are bona fide model to study understand response SCI mechanisms underlying successful regenerative processes. This type research lead identification potential targets therapeutic intervention. article describes how perform tadpole spinal transection, husbandry, surgery, postsurgery care, test evaluation. method be applied elucidating different steps regeneration by studying cellular, molecular, genetic mechanisms, well histological evolution during regeneration.